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abstract::Cell-surface glycans are functional docking sites for tissue lectins such as the members of the galectin family. This interaction triggers a wide variety of responses; hence, there is a keen interest in defining its structural features. Toward this aim, we have used enzyme-linked lectinosorbent (ELLSA) and inhibition ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj102
更新日期:2006-06-01 00:00:00
abstract::Biosynthesis and functions of human milk oligosaccharides (HMO) are not well known. A typical housekeeping enzyme, beta1,4-galactosyltransferase, links galactose to glucose to form lactose which is then used as backbone for the assembly of HMO. We investigated whether milk lactose and HMO may be labeled in vivo by an ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj092
更新日期:2006-06-01 00:00:00
abstract::Glycomics-an integrated approach to study structure-function relationships of complex carbohydrates (or glycans)-is an emerging field in this age of post-genomics. Realizing the importance of glycomics, many large scale research initiatives have been established to generate novel resources and technologies to advance ...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwj080
更新日期:2006-05-01 00:00:00
abstract::The transcriptional regulation mechanisms involved in the up-regulation of Fas-induced GD3 synthase gene have not yet been elucidated. 5'-Rapid amplification of cDNA end (5'-RACE) using mRNA prepared from Fas-induced Jurkat T cells revealed the presence of multiple transcription start sites of human GD3 synthase gene,...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj087
更新日期:2006-05-01 00:00:00
abstract::Lysosomal alpha-mannosidase is a broad specificity exoglycosidase involved in the ordered degradation of glycoproteins. The bovine enzyme is used as an important model for understanding the inborn lysosomal storage disorder alpha-mannosidosis. This enzyme of about 1,000 amino acids consists of five peptide chains, nam...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj081
更新日期:2006-05-01 00:00:00
abstract::Xylosylated and core alpha1,3-fucosylated N-glycans from plants are immunogenic, and they play a still obscure role in allergy and in the field of plant-made protein pharmaceuticals. We immunized mice to generate monoclonal antibodies (mAbs) binding plant N-glycans specifically via the epitope containing either the xy...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj071
更新日期:2006-04-01 00:00:00
abstract::Serglycin with a green fluorescent protein tag (SG-GFP) expressed in epithelial Madin-Darby canine kidney cells is secreted mainly (85%) into the apical medium, but the glycosaminoglycan (GAG) chains on the SG-GFP protein core secreted basolaterally (15%) carry most of the sulfate added during biosynthesis (Tveit et a...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj075
更新日期:2006-04-01 00:00:00
abstract::Isolation of glycopeptides utilizing hydrogen bonding between glycopeptide glycans and a carbohydrate-gel matrix in the organic phase is useful for site-specific characterization of oligosaccharides of glycoproteins, when combined with mass spectrometry. In this study, recovery of glycopeptides was improved by includi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj019
更新日期:2005-12-01 00:00:00
abstract::Glycolipids are important components of almost all biological membranes. They possess unique properties that have only been incompletely characterized so far. The plant glycolipid digalactosyldiacylglycerol (DGDG) strongly influences the physical behavior of phospholipid model membranes in both the dry and hydrated st...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj001
更新日期:2005-11-01 00:00:00
abstract::Many mannose-binding proteins inhibit divergent strains of human immunodeficiency virus type 1 (HIV-1) in in vitro models of viral infectivity, suggesting that targeting mannose residues in vaccine applications might offset the strain restriction typically observed in antibody responses to HIV vaccine preparations. Co...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi083
更新日期:2005-10-01 00:00:00
abstract::Nonreducing O-linked oligosaccharides were obtained from the peptidorhamnomannan of mycelia of Pseudallescheria boydii by alkaline beta-elimination under reducing conditions. They were separated by gel filtration chromatography to give three oligosaccharide fractions. The major oligosaccharide from fraction 1 was char...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi084
更新日期:2005-10-01 00:00:00
abstract::Polysialic acid (PSA) is thought to attenuate neural cell adhesion molecule (NCAM) adhesion, thereby facilitating neural cell migration and regeneration. Although the expression of PSA has been shown to correlate with the progression of certain tumors such as small cell lung carcinoma, there have been no studies to de...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi071
更新日期:2005-09-01 00:00:00
abstract::The hexosamine pathway (HP) is a biochemical hypothesis recently proposed explaining cellular alterations occurring during diabetic microvascular complications. Diabetic retinopathy is a common microvascular complication of diabetes, and it is known that cell proliferation is severely affected during the development o...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi039
更新日期:2005-06-01 00:00:00
abstract::Rabies glycoprotein (RGP(WT)) contains N-glycosylation sequons at Asn(37), Asn(247), and Asn(319), although Asn(37) is not efficiently glycosylated. To examine N-glycan processing at Asn(247) and Asn(319), full-length glycosylation mutants, RGP(-2-) and RGP(--3), were expressed, and Endo H sensitivity was compared. Wh...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi046
更新日期:2005-06-01 00:00:00
abstract::Core alpha1,6-fucosylation is a conserved feature of animal N-linked oligosaccharides being present in both invertebrates and vertebrates. To prove that the enzymatic basis for this modification is also evolutionarily conserved, cDNAs encoding the catalytic regions of the predicted Caenorhabditis elegans and Drosophil...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi028
更新日期:2005-05-01 00:00:00
abstract::In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). Early in this pathway, a proposed lumenal ER lectin, EDEM, recognizes misfolded glycoproteins in the ER, disengages the nascent molecules fro...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi014
更新日期:2005-04-01 00:00:00
abstract::We report the production of biologically active hyaluronan (HA) oligosaccharides labeled with the fluorophore 2-aminobenzoic acid (2AA). Oligosaccharides from 4 to 40 residues in length were purified to homogeneity by ion exchange chromatography using a logarithmic gradient. Molecular weight and purity characterizatio...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi008
更新日期:2005-03-01 00:00:00
abstract::Intestinal maturation can be influenced by intrinsic factors (glucocorticoid hormones) and by extrinsic factors (resident microflora); their relative roles in ontogeny of mouse intestinal trehalase expression, a marker of general gut development, and of beta1,4-galactosyltransferase (beta GT), a marker of glycosyltran...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi004
更新日期:2005-03-01 00:00:00
abstract::The biosynthesis of HNK-1 carbohydrate is mainly regulated by two glucuronyltransferases (GlcAT-P and GlcAT-S) and a sulfotransferase (HNK-1 ST). To determine how the two glucuronyltransferases are involved in the biosynthesis of the HNK-1 carbohydrate, we prepared soluble forms of GlcAT-P and GlcAT-S fused with the I...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi001
更新日期:2005-02-01 00:00:00
abstract::To facilitate deciphering the information content in the glycome, thin film-coated photoactivatable surfaces were applied for covalent immobilization of glycans, glycoconjugates, or lectins in microarray formats. Light-induced immobilization of a series of bacterial exopolysaccharides on photoactivatable dextran-coate...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh143
更新日期:2005-01-01 00:00:00
abstract::Artocarpus lakoocha agglutinin (ALA), isolated from the seeds of A. lakoocha fruit, is a galactose-binding lectin and a potent mitogen of T and B cells. Knowledge obtained from previous studies on the affinity of ALA was limited to molecular and submolecular levels of Galbeta1-->3GalNAc (T) and its derivatives. In the...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh144
更新日期:2005-01-01 00:00:00
abstract::Mouse gene knockout studies have provided unimpeachable evidence of immune-relevant functions for several sialyltransferase enzymes including ST6Gal I, ST3Gal I, and ST3Gal IV. Such studies cannot, however, identify cellular mechanisms for regulating such activities. In this article we provide evidence that murine B l...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh133
更新日期:2004-12-01 00:00:00
abstract::Heparan sulfate/heparin N-deacetylase/N-sulfotransferase-1 (NDST-1) is a critical enzyme involved in heparan sulfate/heparin biosynthesis. This dual-function enzyme modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan. N-sulfation is an absolute requirement for the subsequent epi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh129
更新日期:2004-12-01 00:00:00
abstract::Glycosylation determines essential biological functions of epithelial mucins in health and disease. We report on the influence of glycosylation of the immunodominant DTR motif of MUC1 on its antigenicity. Sets of novel glycopeptides were synthesized that enabled us to examine sole and combined effects of peptide lengt...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh090
更新日期:2004-08-01 00:00:00
abstract::Vesicular integral protein of 36 kDa (VIP36) is an intracellular lectin recognizing high-mannose type glycans and is highly expressed in salivary glands, especially the parotid gland, which secretes alpha-amylase in large quantities. Here immunoelectron microscopy demonstrated that VIP36 was primarily localized to sec...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh082
更新日期:2004-08-01 00:00:00
abstract::Sequestration of Plasmodium falciparum-infected red blood cells (IRBCs) in the human placenta is mediated by chondroitin 4-sulfate (C4S). A cytoadherence assay using chondroitin sulfate proteoglycans (CSPGs) is widely used for studying C4S-IRBC interactions. Bovine tracheal chondroitin sulfate A (CSA) preparation lack...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh077
更新日期:2004-07-01 00:00:00
abstract::Cross-reactive carbohydrate determinants of plants are essentially a mixture of N-glycans containing beta1,2-xylose and core alpha1,3-fucose, the latter also found in insect glycoproteins. To determine the relative contributions of these two sugar residues to antibody binding, we prepared an array of glycomodified for...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh058
更新日期:2004-05-01 00:00:00
abstract::We expressed recombinant Cys subdomains in COS-7 cells to examine the role of this highly conserved protein domain in mucin biosynthesis. The entire Cys1 and Cys5 and Cys1 and Cys3 subdomains in MUC5AC and MUC5B, respectively, each with six carboxyl terminal histidine residues, were pulse-labeled with [(35)S]cysteine/...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh041
更新日期:2004-04-01 00:00:00
abstract::Presently almost no information is available on the oligosaccharide structure of the glycoproteins secreted from the methylotrophic yeast Hansenula polymorpha, a promising host for the production of recombinant proteins. In this study, we analyze the size distribution and structure of N-linked oligosaccharides attache...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh030
更新日期:2004-03-01 00:00:00
abstract::beta1-6 GlcNAc branching, a product of N-acetylglucosaminyltransferase V (GnT-V), is a key structure that is associated with malignant transformations and cancer metastasis. Although a number of reports concerning tumor metastasis-related glycoproteins that contain beta1-6 GlcNAc branching have appeared, the precise f...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh013
更新日期:2004-02-01 00:00:00
abstract::A rapid and reproducible method was developed to detect and quantify carbohydrate-mediated cell adhesion to glycans arrayed on glass slides. Monosaccharides and oligosaccharides were covalently attached to glass slides in 1.7-mm-diameter spots (200 spots/slide) separated by a Teflon gasket. Primary chicken hepatocytes...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh022
更新日期:2004-02-01 00:00:00
abstract::A rat pheochromocytoma cell line (PC12), when transfected with beta1,4-N-acetylglucosaminyltransferase III (GnT-III), which catalyzes the formation of a bisecting GlcNAc structure in N-glycans, resulted in the suppression of neurite outgrowth induced by costimulation of epidermal growth factor (EGF) and integrins. The...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh016
更新日期:2004-02-01 00:00:00
abstract::Multivalency in lectins is a phenomenon that has been discussed at considerable length. The structural basis for the role of multivalency in garlic lectin has been investigated here through computational studies. Biochemical studies have shown that the binding affinity of garlic lectin for high mannose oligosaccharide...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg095
更新日期:2003-11-01 00:00:00
abstract::Heparan sulfate 3-O-sulfotransferase transfers sulfate to the 3-OH position of a glucosamine to generate 3-O-sulfated heparan sulfate (HS), which is a rare component in HS from natural sources. We previously reported that 3-O- sulfotransferase isoform 5 (3-OST-5) generates both an antithrombin-binding site to exhibit ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg101
更新日期:2003-11-01 00:00:00
abstract::Chitin oligosaccharides (COs) play a role in plant development and are presumed to affect body plan formation during vertebrate embryogenesis. The mechanisms of COs recognition and cellular processes underlying embryonic development are still not understood. We analyze the possible link with the mitogen-activated prot...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg103
更新日期:2003-10-01 00:00:00
abstract::The synthesis of the common and well-documented Siaalpha 2,6 to Galbeta 1,4GlcNAc structure (Sia6LacNAc) is principally mediated by the sialyltransferase ST6Gal I, which is particularly highly expressed in liver, lactating mammary gland, intestinal epithelia of newborn animals, and B cells. Multiple independent promot...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg066
更新日期:2003-08-01 00:00:00
abstract::There has been a recent explosion in the identification of neuromuscular diseases caused by mutations in genes that affect carbohydrate metabolism or protein glycosylation. A number of these findings relate to defects in the glycosylation of alpha dystroglycan. Alpha dystroglycan is an essential component of the dystr...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwg077
更新日期:2003-08-01 00:00:00
abstract::Prostate-specific antigen (PSA) is a glycoprotein secreted by prostate epithelial cells. PSA is currently used as a marker of prostate carcinoma because high levels of PSA are indicative of a tumor situation. However, PSA tests still suffer from a lack of specificity to distinguish between benign prostate hyperplasia ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg041
更新日期:2003-06-01 00:00:00
abstract::Schistosoma mansoni soluble egg antigens (SEAs) are crucially involved in modulating the host immune response to infection by S. mansoni. We report that human dendritic cells bind SEAs through the C-type lectin dendritic cell-specific ICAM-3-grabbing nonintegrin (DC-SIGN). Monoclonal antibodies against the carbohydrat...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg052
更新日期:2003-06-01 00:00:00
abstract::The presence of nonmammalian core alpha(1,3)-fucose and core xylose glyco-epitopes on glycans N-linked to therapeutic glycoproteins produced in plants has raised the question of their immunogenicity in human therapy. We address this question by studying the distribution of these N-glycans in pea, rice, and maize (whic...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg024
更新日期:2003-06-01 00:00:00